ProSci

NOVA1 Antibody

Product Code:
 
PSI-46-694
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
1 / 4
46-694 (0.01ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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4 / 4
46-694 (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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46-694 (0.01ug/ml) staining of Human Breast Cancer lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
46-694 (5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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PSI-46-694-0.1mg0.1mg£626.00
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This product comes from: United States.
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Further Information

Additional Names:
NOVA1, neuro-oncological ventral antigen 1, Nova-1, paraneoplastic Ri antigen, ventral neuron-specific protein 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 50-55kDa band observed in Human Breast Cancer lysates (calculated MW of 51.7kDa according to NP_002506.2). In transfected HEK293 transiently expressing NOVA1 a band of approx. 52kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Heart. Recommended concentration: 5ug/ml.
Background References:
  • Jelen N, Ule J, Zivin M, Darnell RB. Evolution of Nova-dependent splicing regulation in the brain. PLoS Genet. 2007 Oct 12;3(10):1838-47. Epub 2007 Aug 27.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-REMPQNVAKTEPVS
NCBI Gene ID #:
4857
NCBI Official Name:
neuro-oncological ventral antigen 1
NCBI Official Symbol:
NOVA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_002506.2 , NP_006480.2 , NP_006482.1
Protein GI Number:
56549653
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer ,Neuroscience
SPECIFICITY:
This antibody is expected to recognise all three reported isoforms (NP_002506.2 ; NP_006480.2 ; NP_006482.1)
Swissprot #:
P51513