Further Information
PIAS2, protein inhibitor of activated STAT, 2 , MGC102682, MIZ1, PIASX-ALPHA, PIASX-BETA, SIZ2, miz , Msx-interacting-zinc finger, protein inhibitor of activated STAT X, ARIP3
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments gave an approx 90kDa band in lysates of cell line A431after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 68.2kDa according to NP_004662.2. The 90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Testis shows nuclear and extra-nuclear staining in spermatids. Recommended concentration, 3-5ug/ml.
- van den Akker E, Ano S, Shih HM, Wang LC, Pironin M, Palvimo JJ, Kotaja N, Kirsh O, Dejean A, Ghysdael J. FLI-1 functionally interacts with PIASxalpha, a member of the PIAS E3 SUMO ligase family. J Biol Chem. 2005 Nov 11;280(45):38035-46. Epub 2005 Sep 7.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
The immunogen for this antibody is: KEAMKVSSQPCTKIE
9063
protein inhibitor of activated STAT, 2
PIAS2
Homo sapiens
Liquid
NP_775298.1, NP_004662.2
27532984
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
This antibody is expected to recognise both reported isoforms (NP_775298.1 and NP_004662.2). No cross-reactivity expected with PIAS1, 3 and 4.
O75928