Peptide ELISA: antibody detection limit dilution 1:16000.
Western Blot:Approx 40-45kDa band observed in Human Parathyroid gland lysates and also in preliminary testing of Mouse Spleen lysates and lysates of cell line U937 (calculated MW of 40.1kDa according to Human NP_002655.2). Recommended concentration: 0.01-0.3ug/ml. Primary incubation 1 hour at room temperature.
Negative Control: HepG2 ( hepatocellular carcinoma) cell lysate.
Immunohistochemistry:Paraffin embedded Human Vessel (platelets) and Spleen. Recommended concentration: 3.75ug/ml.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes and nuclei of HeLa cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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