Further Information
PTPN11, SHP2, protein tyrosine phosphatase, non-receptor type 11 (Noonan syndrome 1), CFC, NS1, BPTP3, PTP2C, SHP-2, PTP-1D, SH-PTP2, SH-PTP3, Noonan syndrome 1, protein-tyrosine phosphatase 2C, protein tyrosine phosphatase, non-receptor type 11, MGC14433, protein tyrosine phosphatase-2
Peptide ELISA: antibody detection limit dilution 1:128000.
Western Blot:Approx 70kDa band observed in Human Muscle lysates (calculated MW of 68.0kDa according to NP_002825.3). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.
Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HeLa cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
- Saxton TM, Ciruna BG, Holmyard D, Kulkarni S, Harpal K, Rossant J, Pawson T. The SH2 tyrosine phosphatase shp2 is required for mammalian limb development. Nat Genet 2000 Apr;24(4):420-3
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Mouse, Rat, Pig, Cow
The immunogen for this antibody is: C-YENVGLMQQQKSFR
5781
protein tyrosine phosphatase, non-receptor type 11
PTPN11
Homo sapiens
Liquid
NP_002825.3, NP_001317366.1
33356177
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer ,Infectious Disease
.
Q06124