Further Information
ROBO1, DUTT1, roundabout, axon guidance receptor, homolog 1 (Drosophila) , HGNC:10249, FLJ21882, SAX3, axon guidance receptor, roundabout 1, MGC131599, MGC133277
Peptide ELISA: antibody detection limit dilution 1:8000.
Western Blot:An anonymous source observed an approx 250kDa band in Human Endothelial cell lysates at 1ug/ml antibody concentration, thought to be due to glycosylation. Some proteolytic breakdown products were also seen. Primary incubation 1 hour at room temperature.
Immunohistochemistry:Paraffin embedded Human Liver. Recommended concentration: 2ug/ml.
Immunofluorescence: Strong expression of the protein seen in the Golgi and membranes of HeLa cells. Recommended concentration: 10ug/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
- Hannula-Jouppi K, Kaminen-Ahola N, Taipale M, Eklund R, Nopola-Hemmi J, Kaariainen H, Kere J. The Axon Guidance Receptor Gene ROBO1 Is a Candidate Gene for Developmental Dyslexia. PLoS Genet. 2005 Oct;1(4):e50. Epub 2005 Oct 28.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
The immunogen for this antibody is: C-GDVDLSNKINEMK
6091
roundabout, axon guidance receptor, homolog 1 (Drosophila)
ROBO1
Homo sapiens
Liquid
NP_002932.1, NP_598334.1
4506569
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
Not expected to cross-react with ROBO2 or ROBO3.
Q9Y6N7