ProSci

UBR4 Antibody

Product Code:
 
PSI-42-191
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Application:
 
Enzyme-Linked Immunosorbent Assay (ELISA)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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42-191 (5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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42-191 (5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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CodeSizePrice
PSI-42-191-0.1mg0.1mg£626.00
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Further Information

Additional Names:
UBR4, ubiquitin protein ligase E3 component n-recognin 4, FLJ41863, KIAA0462, KIAA1307, RBAF600, ZUBR1, p600, retinoblastoma-associated factor 600, retinoblastoma-associated factor 600-like protein, zinc finger, UBR1 type 1
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 574kDa according to NP_065816.2. Therefore we cannot recommend an optimal concentration and the antibody is an aspiring product. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Spleen shows various types of subnuclear staining in select splenocytes and additional membrane plus cytoplasm staining elsewhere. Recommended concentration, 5-10ug/ml.
Background References:
  • Lennerz V, Fatho M, Gentilini C, Frye RA, Lifke A, Ferel D, Wölfel C, Huber C, Wölfel T, The response of autologous T cells to a human melanoma is dominated by mutated neoantigens. Proceedings of the National Academy of Sciences of the United States of America 2005 Nov 102 (44): 16013-8.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-NTTRATSREEKNLQ
NCBI Gene ID #:
23352
NCBI Official Name:
ubiquitin protein ligase E3 component n-recognin 4
NCBI Official Symbol:
UBR4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_065816.2
Protein GI Number:
82659109
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Transcription ,Signal Transduction
Swissprot #:
Q5T4S7