Host Type:
Caprine (Goat)
Antibody Clonality:
Polyclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry (IHC)
- Western Blot (WB)
Storage:
Aliquot and store at -20˚C. Minimize freezing and thawing.
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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Further Information
VPS35, MEM3, FLJ10752, FLJ13588, FLJ20388, DKFZp434E1211, DKFZp434P1672, vacuolar protein sorting 35 (yeast), maternal-embryonic 3, vacuolar protein sorting 35 homolog (S. cerevisiae), vacuolar protein sorting 35
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 90kDa band observed in Human Brain (Cerebellum), Human Heart and Human Ovary and Mouse Brain lysates (calculated MW of 91.7kDa according to NP_060676.2). Recommended concentration: 1.0-3.0ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Brain for this particular batch.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OScells. Recommended concentration: 10ug/ml.
- Zhang P, Yu L, Gao J, Fu Q, Dai F, Zhao Y, Zheng L, Zhao S. Cloning and characterization of human VPS35 and mouse Vps35 and mapping of VPS35 to human chromosome 16q13-q21. Genomics. 2000 Dec 1;70(2):253-7.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Rat, Cow
The immunogen for this antibody is: C-SPESEGPIYEGLIL
55737
vacuolar protein sorting 35 homolog (S. cerevisiae)
VPS35
Homo sapiens
Liquid
NP_060676.2
17999541
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Cancer
Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.
Q96QK1