ProSci

SMAD4 Antibody

Product Code:
 
PSI-27-474
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SMAD4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
1 / 3
Antibody used in WB on Human HepG2 at 1.25 ug/ml.
2 / 3
3 / 3
Antibody used in WB on Human U2OS.
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Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Antibody used in WB on Human U2OS.

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PSI-27-474-100ul100ul£609.00
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Typical lead time: 14-21 working days.
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Further Information

Additional Names:
SMAD4, JIP, DPC4, MADH4, MYHRS
Application Note:
SMAD4 antibody can be used for detection of SMAD4 by ELISA at 1:312500. SMAD4 antibody can be used for detection of SMAD4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Background:
SMAD4 is one of the Smad family members, which are essential intracellular signalling components of the transforming growth factor-beta (TGF-beta) superfamily. Smad2 and Smad3 are structurally highly similar and mediate TGF-beta signals. Smad4 is distantly related to Smads 2 and 3, and forms a heteromeric complex with Smad2 after TGF-beta or activin stimulation. TGF-beta induces heteromeric complexes of Smads 2, 3 and 4, and their concomitant translocation to the nucleus, which is required for efficient TGF-beta signal transduction
Background References:
  • Kantola, A.K., et al., Gene 363, 142-150 (2005).
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD4.
NCBI Gene ID #:
4089
NCBI Official Name:
SMAD family member 4
NCBI Official Symbol:
SMAD4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60 kDa
Protein Accession #:
NP_005350
Protein GI Number:
4885457
Purification:
Antibody is purified by protein A chromatography method.
Research Area:
Transcription ,Cancer ,Signal Transduction
Swissprot #:
Q13485
User NOte:
Optimal dilutions for each application to be determined by the researcher.