ProSci

Apolipoprotein L4 Antibody

Product Code:
 
PSI-46-807
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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46-807 (0.5ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Detected by chemiluminescence.
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46-807 (0.5ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Detected by chemiluminescence.

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PSI-46-807-0.1mg0.1mg£626.00
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Further Information

Additional Names:
apolipoprotein L4, APOL4, apolipoprotein L, 4, APOL-IV, APOLIV, OTTHUMP00000028937, apolipoprotein L-IV
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 37kDa band observed in Human Placenta lysates (calculated MW of 38.7kDa according to NP_085146.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. Two additional bands of unknown identity were also consistently observed at 50kDa and at 70kDa. The band at 50kDa was successfully blocked by incubation with the immunising peptide. The other band at 70kDa however was not blocked by the immunizing peptide and it is therefore a non-specific signal.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5ug/ml.
Background References:
  • Monajemi H, Fontijn RD, Pannekoek H, Horrevoets AJ. The apolipoprotein L gene cluster has emerged recently in evolution and is expressed in human vascular tissue. Genomics. 2002 Apr;79(4):539-46
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunogen for this antibody is: C-ENLNELTHIHQSLK
NCBI Gene ID #:
80832
NCBI Official Name:
apolipoprotein L, 4
NCBI Official Symbol:
APOL4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_085146.2, NP_663693.1
Protein GI Number:
19923626
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Homeostasis
SPECIFICITY:
This antibody is expected to recognize both reported isoforms (NP_085146.2 and NP_663693.1).
Swissprot #:
Q9BPW4