ProSci

MEPCE Antibody

Product Code:
 
PSI-45-883
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
Aliquot and store at -20˚C. Minimize freezing and thawing.
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45-883 (1ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
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45-883 (1ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

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CodeSizePrice
PSI-45-883-0.1mg0.1mg£626.00
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Further Information

Additional Names:
MEPCE, methylphosphate capping enzyme, BCDIN3, FLJ20257, bin3, bicoid-interacting 3, bin3, bicoid-interacting 3, homolog
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx. 75kDa band observed in Rat Testis lysates (calculated MW of 74.4kDa according to Human NP_062552.2 and 75.3kDa according to Rat XP_222048.4). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Human for this particular batch.Immunohistochemistry:In paraffin embedded Human Testis shows staining of the spermatids. Recommended concentration: 5-10ug/ml.
Background References:
  • Jeronimo C, Forget D, Bouchard A, Li Q, Chua G, Poitras C, Therien C, Bergeron D, Bourassa S, Greenblatt J, Chabot B, Poirier GG, Hughes TR, Blanchette M, Price DH, Coulombe B. Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme. Mol Cell. 2007 Jul 20;27(2):262-74.
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20?C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: KQQRKFQYGNYCK
NCBI Gene ID #:
56257
NCBI Official Name:
methylphosphate capping enzyme
NCBI Official Symbol:
MEPCE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_062552.2
Protein GI Number:
47271406
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Research Area:
Cancer
SPECIFICITY:
.
Swissprot #:
Q7L2J0