Further Information
ZAP70, zeta-chain (TCR) associated protein kinase 70kDa, FLJ17670, FLJ17679, SRK, STD, TZK, ZAP-70, 70 kDa zeta-associated protein, syk-related tyrosine kinase, tyrosine-protein kinase ZAP-70, zeta-chain associated protein kinase, 70kD
Peptide ELISA: antibody detection limit dilution 1:32000.
Western Blot:Approx 70kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 69.9kDa according to NP_001070.2). Recommended concentration: 0.3-1ug/ml.
Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from customer).
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20?C. Minimize freezing and thawing.
500 ug/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
The immunogen for this antibody is: C-SDGYTPEPARITSPD
7535
zeta-chain (TCR) associated protein kinase 70kDa
ZAP70
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Approx 70 kDa
NP_001070.2
31455611
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
This antibody is expected to recognize isoform 1 (NP_001070.2) only.
P43403