Further Information
AT rich interactive domain 1A (SWI-like), RP1-50O24.1, B120, BAF250, BAF250a, BM029, C1orf4, ELD, hELD, MRD14, OSA1, hOSA1, P270, SMARCF1
ARID1A antibody can be used for detection of ARID1A by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
The ARID1A protein is a member of the SWI/SNF family, whose members are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. ARID1A is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin (1). It possesses a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. The C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that ARID1A confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions (2).
- Martens JA and Winston F. Recent advances in understanding chromatin remodeling by Swi/Snf complexes. Curr. Opin. Genet. Dev. 2003; 13:136-42.
- Nie Z, Xue Y, Yang D, et al. A specificity and targeting subunit of a human SWI/SNF family-related chromatin-remodeling complex. Mol. Cell. Biol. 2000; 20:8879-88.
ARID1A antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
ARID1A antibody was raised against an 18 amino acid peptide near the amino terminus of human ARID1A.
The immunogen is located within amino acids 580 - 630 of ARID1A.
8289
AT rich interactive domain 1A (SWI-like)
ARID1A
homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 133, 227, 251 kDa
Observed: 130, 260 kDa
NP_006006
21264565
ARID1A antibody is affinity chromatography purified via peptide column.
Homeostasis,Signal Transduction
ARID1A antibody is human, mouse and rat reactive. Multiple isoforms of ARID1A protein are known to exist.
O14497
Optimal dilutions for each application to be determined by the researcher.