ProSci

ADAM29 Antibody

Product Code:
 
PSI-8183
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
 
ADAM29 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
1 / 3
Western blot analysis of ADAM29 in human bladder tissue lysate with ADAM29 antibody at (A) 1 and (B) 2 μg/ml.
2 / 3
Immunohistochemistry of ADAM29 in human testis tissue with ADAM29 antibody at 5 μg/mL.
3 / 3
Immunofluorescence of ADAM29 in human testis tissue with ADAM29 antibody at 20 μg/mL.
❮❯

Western blot analysis of ADAM29 in human bladder tissue lysate with ADAM29 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of ADAM29 in human testis tissue with ADAM29 antibody at 5 μg/mL.
Immunofluorescence of ADAM29 in human testis tissue with ADAM29 antibody at 20 μg/mL.

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CodeSizePrice
PSI-8183-0.02mg0.02mg£150.00
Quantity:
PSI-8183-0.1mg0.1mg£449.00
Quantity:
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Further Information

Additional Names:
Disintegrin and metalloproteinase domain-containing protein 29, ADAM metallopeptidase domain 29, CT73, SVPH1
Application Note:
ADAM29 antibody can be used for detection of ADAM29 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Background:
The ADAM (a disintegrin and metalloprotease) protein family plays a role in cell-cell and cell-matrix interface related processes, including fertilization, muscle fusion, secretion of TNFalpha (tumor necrosis factor alpha), and modulation of the neurogenic function of Notch and Delta (1-3). ADAM29 contains one disintegrin domain, one EGF-like domain and one peptidase M12B domain (2). It is highly expressed in testes (3) and may be involved in spermatogenesis and fertilization (4,5).
Background References:
  • Wang F, Xu R, Zhu P, et al. Preliminarily functional analysis of a cloned novel human gene ADAM29. Sci. China C. Life Sci. 2001; 44392-9.
  • Xu R, Cai J, Xu T, et al. Molecular cloning and mapping of a novel ADAM gene (ADAM29) to human chromosome 4. Genomics 1999; 62:537-9.
  • Cerretti DP, DuBose RF, Black RA, et al. Isolation of two novel metalloproteinase-disintegrin (ADAM) cDNAs that show testis-specific gene expression. Biochem. Biophys. Res. Commun. 1999; 263:810-5.
  • Oppezzo P, Vasconcelos Y, Settegrana C, et al. The LPL/ADAM29 expression ratio is a novel prognosis indicator in chronic lymphocytic leukemia. Blood 2005; 106:650-7.
Buffer:
ADAM29 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
ADAM29 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ADAM29.

The immunogen is located within amino acids 710 - 760 of ADAM29.
NCBI Gene ID #:
11086
NCBI Official Name:
ADAM metallopeptidase domain 29
NCBI Official Symbol:
ADAM29
NCBI Organism:
homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 76 kDa

Observed: 80 kDa
Protein Accession #:
NP_055084
Protein GI Number:
73765552
Purification:
ADAM29 antibody is affinity chromatography purified via peptide column.
Research Area:
Homeostasis,Stem Cell
SPECIFICITY:
ADAM29 antibody is human specific. ADAM29 antibody is predicted to not cross-react with other ADAM family proteins.
Swissprot #:
Q9UKF5
User NOte:
Optimal dilutions for each application to be determined by the researcher.