Further Information
Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD
GAPDH antibody can be used for detection of GAPDH by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer?s and Huntington?s disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
- Sirover MA. New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J. Cell. Biochem. 2005; 95:45-52.
- Glyceraldehyde-3-phosphate dehydrogenase, apoptosis, and neurodegenerative diseases. Annu. Rev. Pharmacol. Toxicol. 2005; 45:269-90.
GAPDH antibody is supplied in PBS containing 0.02% sodium azide.
1 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
GAPDH antibody was raised against a 16 amino acid peptide near the carboxy terminus of human GAPDH.
2597
glyceraldehyde-3-phosphate dehydrogenase
GAPDH
homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
Predicted: 37 kDa
Observed: 36 kDa
NP_002037
7669492
GAPDH antibody is Protein A purified.
Signal Transduction,Neuroscience,Homeostasis
GAPDH antibody is human, mouse,and rat reactive.
P04406
Optimal dilutions for each application to be determined by the researcher.