ProSci

LDLR Recombinant Protein

Product Code:
 
PSI-96-509
Product Group:
 
Recombinant Proteins
Supplier:
 
ProSci
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
 

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PSI-96-509-0.1mg0.1mg£765.00
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Further Information

Additional Names:
LDLR, FH, FHC, LDLCQ2
Application Note:
This recombinant protein can be used for WB. For research use only.
Background:
Low-Density Lipoprotein (LDL) Receptor is also known as LDLR, FH, FHC, LDLCQ2, and is a mosaic protein of ~840 amino acids (after removal of signal peptide) that mediates the endocytosis of cholesterol-rich LDL. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants (IDL). It belongs to the Low density lipoprotein receptor gene family. LDL receptor complexes are present in clathrin-coated pits (or buds) on the cell surface, which when bound to LDL-cholesterol via adaptin, are pinched off to form clathrin-coated vesicles inside the cell. This allows LDL-cholesterol to be bound and internalized in a process known as endocytosis and prevents the LDL just diffusing around the membrane surface. This occurs in all nucleated cells (not erythrocytes), but mainly in the liver which removes ~70% of LDL from the circulation. Synthesis of receptors in the cell is regulated by the level of free intracellular cholesterol; if it is in excess for the needs of the cell then the transcription of the receptor gene will be inhibited. LDL receptors are translated by ribosomes on the endoplasmic reticulum and are modified by the Golgi apparatus before travelling in vesicles to the cell surface. LDL is directly involved in the development of atherosclerosis, due to accumulation of LDL-cholesterol in the blood. Atherosclerosis is the process responsible for the majority of cardiovascular diseases.
Background References:
  • Yamamoto T, et al., 1984, Cell 39 : 27?38.
  • Nykjaer A, Willnow TE, 2002, Trends Cell Biol. 12 (6): 273?80.
  • Barrett P.H., Watts G.F. 2002, Atherosclerosis. Supplements. 2: 1-4.
  • Nomura S. et al., 2004, Gene. Therapy. 11: 1540-1548.
Biological Activity:
(1) Measured by its binding ability in a functional ELISA. Immobilized Human LDL R, His Tag at 10 ug/mL (100 ul/well) can bind Biotinylated Human PCSK9 with a linear range of 0.01-0.1 ug/mL. (2) Measured by its binding ability in a functional ELISA. Immobilized Human LDL R, His Tag at 10 ug/mL (100 ul/well) can bind Biotinylated Human (D374Y) PCSK9 with a linear range of 0.02-0.5 ug/mL.
Buffer:
PBS, pH7.4
Fusion Tag:
His Tag
NCBI Gene ID #:
3949
NCBI Official Name:
low density lipoprotein receptor
NCBI Official Symbol:
LDLR
NCBI Organism:
Homo sapiens
Physical State:
Lyophilized
Predicted Molecular Weight:
86 kDa
Protein Accession #:
NP_000518.1
Purity:
>90% as determined by SDS-PAGE.
Recombinant Protein Sequence:
Thr32-Gly402
Source:
HEK293 cells
Swissprot #:
P01130

Documents