Sciencell

GeneQuery™ Human B Cell Receptor Signaling Pathway qPCR Array Kit

Product Code:
 
SC-GK020
Product Group:
 
PCR Kits
Supplier:
 
Sciencell
Host Type:
 
Human
Regulatory Status:
 
RUO
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SC-GK0201 kit£297.00
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Further Information

Description:
ScienCell's GeneQuery™ Human B Cell Receptor Signaling Pathway qPCR Array Kit (GQH-BRS) is designed to facilitate gene expression profiling of 88 key genes involved in B cell receptor signaling pathway. The B cell receptor signaling pathway is important for antibody production, B cell proliferation and differentiation. Brief examples of how included genes may be grouped according to their functions are shown below:
  • B cell receptor complex components: - CD79A, CD79B
  • Co-inhibitory receptors: - CD22, CD72, FCGR2B
  • Co-stimulators: - CD81, CR2, IFITM1
  • Expression adaptor proteins: - BLNK, GAB2, GRB2, CARD11
  • Kinases: - LYN, SYK, PI3Ks
  • Phosphatases: - INPP5D, PTPN6
  • MAPK pathway: - KRAS, HRAS, NRAS, RAF1, MAP2K1, MAPK1, MAPK3
  • PI3K/AKT pathway: - PI3Ks, AKT1, AKT2
  • IKK/NFKB pathway: - NFKBs, PRKCB, PLCG2, DAG1, BCL10, CARD11, MAP3K7, MALT1, CHUK, IKBKB, IKBKG
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg^2+ , and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
Extra Description:
This kit is designed to facilitate gene expression profiling of 88 key genes involved in B cell receptor signaling pathway.