HematoGro Medium

HematoGro Medium (HemGM), when used with HematoGro Supplement (HemGS, Cat #5552) is a complete medium designed for short-term maintenance of hematopoietic cells in vitro, including bone marrow mononuclear and peripheral blood cells. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, hormones, organic and inorganic compounds and bovine serum albumin. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. This medium does not contain serum, growth factors or cytokines. Supplementation with additional factors may be used to support the proliferation or differentiation of select cell subpopulations.

HemGM consists of 500 ml of basal medium, 5 ml of HematoGro Supplement (HemGS; Cat #5552) and 5 ml of penicillin/streptomycin solution (P/S; Cat. #0503). Note: HemGS and P/S are not pre-mixed in HemGM; they must be added separately to make the complete HemGM.