ProSci

MAGEA12 Antibody

Product Code:
 
PSI-62-151
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in K562 cell line lysates (35ug/lane).
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MAGEA12 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in K562 cell line lysates (35ug/lane).
MAGEA12 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-62-151-400ul400ul£626.00
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This product comes from: United States.
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Further Information

Additional Names:
Melanoma-associated antigen 12, Cancer/testis antigen 112, CT112, MAGE-12 antigen, MAGE12F antigen, MAGEA12, MAGE12
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
MAGEA12 is a member of the melanoma antigen gene (MAGE) family. The proteins of this family are tumor-specific antigens that can be recognized by autologous cytolytic T lymphocytes. This gene is expressed in various tumors and tumor cell lines from different tissue origins, but not detected in normal tissues, except testis. The function of this gene is unknown. This and other MAGE genes form a gene cluster at chromosome Xq28 region.
Background References:
  • Mallon, A.M., et al., Genome Res. 10(6):758-775 (2000).
  • Rogner, U.C., et al., Genomics 29(3):725-731 (1995).
  • Ding, M., et al., Biochem. Biophys. Res. Commun. 202(1):549-555 (1994).
  • De Smet, C., et al., Immunogenetics 39(2):121-129 (1994).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MAGEA12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-108 amino acids from the N-terminal region of human MAGEA12.
NCBI Gene ID #:
4111
NCBI Official Name:
Melanoma-associated antigen 12
NCBI Official Symbol:
MAGEA12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
35 kDa
Protein Accession #:
P43365
Protein GI Number:
14286145
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer
Swissprot #:
P43365
User NOte:
Optimal dilutions for each application to be determined by the researcher.