Antibody Isotype:
Rabbit Ig
Antibody Clonality:
Polyclonal
Applications:- Flow Cytometry
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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![Western Blot at 1:2000 dilution Lane 1: HT-29 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Raji whole cell lysate Lysates/proteins at 20 ug per lane. Western Blot at 1:2000 dilution Lane 1: HT-29 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Raji whole cell lysate Lysates/proteins at 20 ug per lane.](https://www.prosci-inc.com/static-images/56-286_1.jpg)
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![Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis. Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.](https://www.prosci-inc.com/static-images/56-286_2.jpg)
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This product comes from:
United States.
Typical lead time:
14-21 working days.
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- Further Information
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Further Information
Retinoblastoma-like protein 2, 130 kDa retinoblastoma-associated protein, p130, Retinoblastoma-related protein 2, RBR-2, pRb2, RBL2, RB2
For WB starting dilution is: 1:2000
For FACS starting dilution is: 1:10~50
RBL2 is a key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. RBL2 may act as a tumor suppressor.
- Schaffer, B.E., et al. Cancer Res. 70(10):3877-3883(2010)
- Barrow-Laing, L., et al. Virology 400(2):233-239(2010)
- Lu, F., et al. J. Virol. 84(6):2697-2706(2010)
- Jowett, J.B., et al. Diabetes 59(3):726-732(2010)
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Predicted species reactivity based on immunogen sequence: Mouse, Rat
This RBL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 165-194 amino acids from the N-terminal region of human RBL2.
5934
Retinoblastoma-like protein 2
RBL2
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
128 kDa
Q08999
116242746
This antibody is purified through a protein A column, followed by peptide affinity purification.
Cancer,Cell Cycle
Q08999
Optimal dilutions for each application to be determined by the researcher.