ProSci

CDK2 Antibody

Product Code:
 
PSI-62-964
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis of anti-CDK2 Antibody in Jurkat cell line lysates (35ug/lane)
2 / 3
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CDK2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3

Western blot analysis of anti-CDK2 Antibody in Jurkat cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CDK2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-62-964-400ul400ul£626.00
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Further Information

Additional Names:
Cyclin-dependent kinase 2, Cell division protein kinase 2, p33 protein kinase, CDK2, CDKN2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
CDK2 is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation.
Background References:
  • Moshinsky, D.J., et al., Biochem. Biophys. Res. Commun. 310(3):1026-1031 (2003).
  • Chow, J.P., et al., J. Biol. Chem. 278(42):40815-40828 (2003).
  • O'Nions, J., et al., Oncogene 22(46):7181-7191 (2003).
  • Yun, J., et al., J. Biol. Chem. 278(38):36966-36972 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Hamster, Mouse, Rat, Xenopus
Immunogen:
This CDK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-260 amino acids from the C-terminal region of human CDK2.
NCBI Gene ID #:
1017
NCBI Official Name:
Cyclin-dependent kinase 2
NCBI Official Symbol:
CDK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
34 kDa
Protein Accession #:
P24941
Protein GI Number:
116051
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle
Swissprot #:
P24941
User NOte:
Optimal dilutions for each application to be determined by the researcher.