ProSci

PTTG1 Antibody

Product Code:
 
PSI-62-848
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western Blot at 1:500 dilution + Daudi whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 3
Immunofluorescent analysis of U251 cells, using PTTG1 Antibody . Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).
3 / 3
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Western Blot at 1:500 dilution + Daudi whole cell lysate Lysates/proteins at 20 ug per lane.
Immunofluorescent analysis of U251 cells, using PTTG1 Antibody . Antibody was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).

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PSI-62-848-400ul400ul£662.00
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Further Information

Additional Names:
Securin, Esp1-associated protein, Pituitary tumor-transforming gene 1 protein, Tumor-transforming protein 1, hPTTG, PTTG1, EAP1, PTTG, TUTR1
Application Note:
For WB starting dilution is: 1:500

For IF starting dilution is: 1:100

For IHC-P starting dilution is: 1:50~100
Background:
PTTG1 is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The protein has transforming activity in vitro and tumorigenic activity in vivo, and is highly expressed in various tumors. This protein contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the protein can act as a transactivation domain. It is mainly a cytosolic protein, although it partially localizes in the nucleus.
Background References:
  • Li,T.,Genet Genomics 36 (6), 335-342 (2009)
  • Yan,S., Cancer Res. 69 (8), 3283-3290 (2009)
  • Chesnokova,V., Horm. Res. 71 SUPPL 2, 82-87 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PTTG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-45 amino acids from the N-terminal region of human PTTG1.
NCBI Gene ID #:
9232
NCBI Official Name:
Securin
NCBI Official Symbol:
PTTG1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
22 kDa
Protein Accession #:
O95997
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle
Swissprot #:
O95997
User NOte:
Optimal dilutions for each application to be determined by the researcher.