Antibody Isotype:
Rabbit Ig
Antibody Clonality:
Polyclonal
Applications:- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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This product comes from:
United States.
Typical lead time:
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Further Information
Apoptosis-inducing factor 1, mitochondrial, 111-, Programmed cell death protein 8, AIFM1, AIF, PDCD8
For IF starting dilution is: 1:200
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
AIFM1 is a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this protein induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9.
- References for protein:
- Daugas,E., et.al., FASEB J. 14 (5), 729-739 (2000)
- Schulthess,F.T., et.al., PLoS ONE(2), E4394 (2009)
- References for U251 cell line:
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
This AIFM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-98 amino acids from the N-terminal region of human AIFM1.
9131
Apoptosis-inducing factor 1, mitochondrial
AIFM1
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
67 kDa
O95831
This antibody is purified through a protein A column, followed by peptide affinity purification.
Cancer,Cell Cycle,Immunology,Obesity,Neuroscience
O95831
Optimal dilutions for each application to be determined by the researcher.