ProSci

GATA4 Antibody

Product Code:
 
PSI-57-445
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of anti-GATA4 Antibody in CEM cell line lysates (35ug/lane).
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Western blot analysis of GATA4 using rabbit polyclonal GATA4 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GATA4 gene (Lane 2).
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Western blot analysis of anti-GATA4 Antibody in CEM cell line lysates (35ug/lane).
Western blot analysis of GATA4 using rabbit polyclonal GATA4 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GATA4 gene (Lane 2).

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PSI-57-445-400ul400ul£626.00
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Further Information

Additional Names:
Transcription factor GATA-4, GATA-binding factor 4, GATA4
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
GATA4 is a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in the gene encoding GATA4 have been associated with cardiac septal defects.
Background References:
  • Rajagopal,S.K., J. Mol. Cell. Cardiol. 43 (6), 677-685 (2007)
  • Philips,A.S., J. Biol. Chem. 282 (34), 24915-24927 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Mouse, Rat
Immunogen:
This GATA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-328 amino acids from the C-terminal region of human GATA4.
NCBI Gene ID #:
2626
NCBI Official Name:
Transcription factor GATA-4
NCBI Official Symbol:
GATA4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
P43694
Protein GI Number:
215274105
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle,Signal Transduction
Swissprot #:
P43694
User NOte:
Optimal dilutions for each application to be determined by the researcher.