ProSci

CD38 Antibody

Product Code:
 
PSI-61-570
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 7
Western blot analysis of lysate from 293T cell line, using CD38 Antibody at 1:1000.
2 / 7
Western blot analysis of lysate from RPMI8226 cell line, using CD38 Antibody at 1:1000.
3 / 7
4 / 7
Immunofluorescence analysis of CD38 Antibody with paraffin-embedded human hepatocarcinoma tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
5 / 7
Flow cytometric analysis of HepG2 cells using CD38 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
6 / 7
Confocal immunofluorescent analysis of CD38 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
7 / 7
Western blot analysis of lysate from RPMI 8226 cell line, using CD38 Antibody at 1:1000.
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Western blot analysis of lysate from 293T cell line, using CD38 Antibody at 1:1000.
Western blot analysis of lysate from RPMI8226 cell line, using CD38 Antibody at 1:1000.
Immunofluorescence analysis of CD38 Antibody with paraffin-embedded human hepatocarcinoma tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
Flow cytometric analysis of HepG2 cells using CD38 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of CD38 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of lysate from RPMI 8226 cell line, using CD38 Antibody at 1:1000.

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PSI-61-570-400ul400ul£626.00
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Further Information

Additional Names:
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1, 2'-phospho-ADP-ribosyl cyclase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-cyclic-ADP-ribose transferase, ADP-ribosyl cyclase 1, ADPRC 1, Cyclic ADP-ribose hydrolase 1, cADPr hydrolase 1, T10, CD38, CD38
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
CD38 is a novel multifunctional ectoenzyme widely expressed in cells and tissues especially in leukocytes. CD38 also functions in cell adhesion,signal transduction and calcium signaling.
Background References:
  • Horenstein,A.L., Mol. Med. 15 (3-4), 76-84 (2009) Liu,Q., Biochemistry 47 (52), 13966-13973 (2008) Liu,Q., Chem. Biol. 15 (10), 1068-1078 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This CD38 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-270 amino acids from the C-terminal region of human CD38.
NCBI Gene ID #:
952
NCBI Official Name:
ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
NCBI Official Symbol:
CD38
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
34 kDa
Protein Accession #:
P28907
Protein GI Number:
55977782
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Immunology,Obesity,Signal Transduction
Swissprot #:
P28907
User NOte:
Optimal dilutions for each application to be determined by the researcher.