ProSci

JUN Antibody

Product Code:
 
PSI-60-744
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in PC-3, U87-MG cell line lysates (35ug/lane).This demonstdetected the JUN protein (arrow).

Western blot analysis in PC-3, U87-MG cell line lysates (35ug/lane).This demonstdetected the JUN protein (arrow).

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PSI-60-744-400ul400ul£626.00
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Further Information

Additional Names:
Transcription factor AP-1, Activator protein 1, AP1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, JUN
Application Note:
For WB starting dilution is: 1:1000
Background:
JUN interacts directly with specific target DNA sequences to regulate gene expression. Jun recognizes the AP-1 consensus sequence TGACTCA, a response element that confers sensitivity to one of the tumor-promoting Phorbol esters, 12-O-tetradecanoyl-phorbol-13-acetate (see also: TRE, TPA response element). Jun itself forms homodimers or heterodimers with junD and junB and also interacts with the oncogene product fos, forming jun-fos heterodimers.
Background References:
  • Fujita,S., J. Mol. Biol. 378 (3), 492-504 (2008)
  • Gan,X.Q.,Cell Biol. 180 (6), 1087-1100 (2008)
  • Yogev,O., Cancer Res. 68 (5), 1398-1406 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This JUN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-70 amino acids from human JUN.
NCBI Gene ID #:
3725
NCBI Official Name:
Transcription factor AP-1
NCBI Official Symbol:
JUN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
36 kDa
Protein Accession #:
P05412
Protein GI Number:
135298
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Immunology,Signal Transduction
Swissprot #:
P05412
User NOte:
Optimal dilutions for each application to be determined by the researcher.