Further Information
Serine/threonine-protein kinase A-Raf, Proto-oncogene A-Raf, Proto-oncogene A-Raf-1, Proto-oncogene Pks, ARAF, ARAF1, PKS, PKS2
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
- Wu, X., et al., J. Biol. Chem. 271(6):3265-3271 (1996).
- Lee, J.E., et al., Genomics 20(1):43-55 (1994).
- Popescu, N.C., et al., Oncogene 4(4):517-519 (1989).
- Beck, T.W., et al., Nucleic Acids Res. 15(2):595-609 (1987).
Supplied in PBS with 0.09% (W/V) sodium azide.
batch dependent
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
This A-RAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-286 amino acids from the Central region of human A-RAF.
369
Serine/threonine-protein kinase A-Raf
ARAF
Homo sapiens
Liquid
PREDICTED MOLECULAR WEIGHT:
68 kDa
P10398
1730068
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Cancer,Neuroscience,Signal Transduction
P10398
Optimal dilutions for each application to be determined by the researcher.