ProSci

Leptin Receptor Antibody

Product Code:
 
PSI-62-143
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the LEPR antibody detected the LEPR protein (arrow).
2 / 3
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the LEPR antibody detected the LEPR protein (arrow).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-62-143-400ul400ul£626.00
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Further Information

Additional Names:
Leptin receptor, LEP-R, HuB219, OB receptor, OB-R, CD295, LEPR, DB, OBR
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:50~100
Background:
Leptin, an adipocyte-specific hormone, regulates adipose-tissue mass through hypothalamic effects on satiety and energy expenditure by acting through the leptin receptor (LEPR). LEPR is a single-transmembrane-domain receptor of the cytokine receptor family that is identical to the mouse diabetes (db) gene product. During weight loss, leptin levels decrease, whereas soluble LEPR levels and the receptor bound fraction of leptin increases. The presence of LEPR in the absorptive cells of the small intestine suggests that leptin may have a physiological role in the regulation of nutrient absorption.
Background References:
  • Schroth, M., et al., J. Clin. Endocrinol. Metab. 88(11):5497-5501 (2003).
  • Couturier, C., et al., J. Biol. Chem. 278(29):26604-26611 (2003).
  • Gavrila, A., et al., J. Clin. Endocrinol. Metab. 88(6):2838-2843 (2003).
  • Yannakoulia, M., et al., J. Clin. Endocrinol. Metab. 88(4):1730-1736 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Leptin Receptor (LEPR) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-52 amino acids from the N-terminal region of human Leptin Receptor (LEPR).
NCBI Gene ID #:
3953
NCBI Official Name:
Leptin receptor
NCBI Official Symbol:
LEPR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
132 kDa
Protein Accession #:
P48357
Protein GI Number:
116242617
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Obesity,Neuroscience,Signal Transduction
Swissprot #:
P48357
User NOte:
Optimal dilutions for each application to be determined by the researcher.