ProSci

AMPK alpha Antibody

Product Code:
 
PSI-62-772
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western Blot at 1:2000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 3
Western blot analysis of PRKAA1 using rabbit polyclonal PRKAA1 Antibody (S246) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKAA1 gene (Lane 2).
3 / 3
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Western Blot at 1:2000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of PRKAA1 using rabbit polyclonal PRKAA1 Antibody (S246) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKAA1 gene (Lane 2).

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PSI-62-772-400ul400ul£626.00
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Further Information

Additional Names:
5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, Tau-protein kinase PRKAA1, PRKAA1, AMPK1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
PRKAA1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.
Background References:
  • Pang,T., J. Biol. Chem. 282 (1), 495-506 (2007)
  • Crawford,R.M., Cell. Signal. 18 (10), 1595-1603 (2006)
  • Crawford,R.M., Mol. Cell. Biol. 26 (15), 5921-5931 (2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This AMPK alpha(PRKAA1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 474-502 amino acids from human AMPK alpha(PRKAA1).
NCBI Gene ID #:
5562
NCBI Official Name:
5'-AMP-activated protein kinase catalytic subunit alpha-1
NCBI Official Symbol:
PRKAA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
64 kDa
Protein Accession #:
Q13131
Protein GI Number:
254763436
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Neuroscience,Signal Transduction,Autophagy
Swissprot #:
Q13131
User NOte:
Optimal dilutions for each application to be determined by the researcher.