ProSci

ARNT Antibody

Product Code:
 
PSI-57-640
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Fluorescent image of A549 cell stained with hARNT-V528.A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with hARNT primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).hARNT immunoreactivity is localized to Nucleus significantly.
2 / 2
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
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Fluorescent image of A549 cell stained with hARNT-V528.A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with hARNT primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).hARNT immunoreactivity is localized to Nucleus significantly.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

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PSI-57-640-400ul400ul£626.00
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Further Information

Additional Names:
Aryl hydrocarbon receptor nuclear translocator, ARNT protein, Class E basic helix-loop-helix protein 2, bHLHe2, Dioxin receptor, nuclear translocator, Hypoxia-inducible factor 1-beta, HIF-1-beta, HIF1-beta, ARNT, BHLHE2
Application Note:
For IF starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
The aryl hydrocarbon (Ah) receptor is involved in the induction of several enzymes that participate in xenobiotic metabolism. The ligand-free, cytosolic form of the Ah receptor is complexed to heat shock protein 90. Binding of ligand, which includes dioxin and polycyclic aromatic hydrocarbons, results in translocation of the ligand-binding subunit only to the nucleus. Induction of enzymes involved in xenobiotic metabolism occurs through binding of the ligand-bound Ah receptor to xenobiotic responsive elements in the promoters of genes for these enzymes. This gene encodes a protein that forms a complex with the ligand-bound Ah receptor, and is required for receptor function. The encoded protein has also been identified as the beta subunit of a heterodimeric transcription factor, hypoxia-inducible factor 1. A t(1;12)(q21;p13) translocation, which results in a TEL-ARNT fusion protein, is associated with acute myeloblastic leukemia. Alternative splicing results in multiple transcript variants.
Background References:
  • Otsubo, K., et al. Cancer Genet. Cytogenet. 202(1):22-26(2010)
  • Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
  • Jugessur, A., et al. PLoS ONE(7), E11493 (2010) :
  • Kewley, R.J., et al. Biochem. Biophys. Res. Commun. 338(1):660-667(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This ARNT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 513-544 amino acids from the Central region of human ARNT.
NCBI Gene ID #:
405
NCBI Official Name:
Aryl hydrocarbon receptor nuclear translocator
NCBI Official Symbol:
ARNT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
87 kDa
Protein Accession #:
P27540
Protein GI Number:
114163
Purification:
This antibody is purified through a protein G column, followed by dialysis against PBS.
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
P27540
User NOte:
Optimal dilutions for each application to be determined by the researcher.