ProSci

AGL Antibody

Product Code:
 
PSI-61-376
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 4
Western Blot at 1:8000 dilution + human skeletal muscle lysate Lysates/proteins at 20 ug per lane.
2 / 4
Western blot using anti-AGL antibody at 1:1000 dilution. A total of 20 ug of lysates was loaded for each tissue. Data courtesy of Dr. Alan Cheng, Department of Internal Medicine, Life Sciences Institute, University of Michigan Medical Center, Ann Arbor, Michigan.
3 / 4
4 / 4
Confocal immunofluorescent analysis of AGL Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
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Western Blot at 1:8000 dilution + human skeletal muscle lysate Lysates/proteins at 20 ug per lane.
Western blot using anti-AGL antibody at 1:1000 dilution. A total of 20 ug of lysates was loaded for each tissue. Data courtesy of Dr. Alan Cheng, Department of Internal Medicine, Life Sciences Institute, University of Michigan Medical Center, Ann Arbor, Michigan.
Confocal immunofluorescent analysis of AGL Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

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PSI-61-376-400ul400ul£626.00
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Further Information

Additional Names:
Glycogen debranching enzyme, Glycogen debrancher, 4-alpha-glucanotransferase, Oligo-1,4-1,4-glucantransferase, Amylo-alpha-1,6-glucosidase, Amylo-1,6-glucosidase, Dextrin 6-alpha-D-glucosidase, AGL, GDE
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
AGL is a glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in the AGL gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing.
Background References:
  • Horinishi, A., et al., J. Hum. Genet. 47(2):55-59 (2002).
  • Bao, Y., et al., Genomics 38(2):155-165 (1996).
  • Yang, B.Z., et al., J. Biol. Chem. 267(13):9294-9299 (1992).
  • Yang-Feng, T.L., et al., Genomics 13(4):931-934 (1992).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This AGL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1479-1510 amino acids from the C-terminal region of human AGL.
NCBI Gene ID #:
178
NCBI Official Name:
Glycogen debranching enzyme
NCBI Official Symbol:
AGL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
175 kDa
Protein Accession #:
P35573
Protein GI Number:
116242491
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Signal Transduction
Swissprot #:
P35573
User NOte:
Optimal dilutions for each application to be determined by the researcher.