ProSci

DKK3 Antibody

Product Code:
 
PSI-58-169
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis of lysates from 293, 293T, A549, HL-60, MCF-7 cell line and mouse brain tissue lysate (from left to right), using DKK3 Antibody (A30) at 1:1000 at each lane.
2 / 3
Formalin-fixed and paraffin-embedded human brain tissue reacted with DKK3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3
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Western blot analysis of lysates from 293, 293T, A549, HL-60, MCF-7 cell line and mouse brain tissue lysate (from left to right), using DKK3 Antibody (A30) at 1:1000 at each lane.
Formalin-fixed and paraffin-embedded human brain tissue reacted with DKK3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-58-169-400ul400ul£662.00
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Further Information

Additional Names:
Dickkopf-related protein 3, Dickkopf-3, Dkk-3, hDkk-3, DKK3, REIC
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, and heart.
Background References:
  • Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003).
  • Tsuji, T., et al., Biochem. Biophys. Res. Commun. 268(1):20-24 (2000).
  • Krupnik, V.E., et al., Gene 238(2):301-313 (1999).
  • Kobayashi, K., et al., Gene 282 (1-2), 151-158 (2002).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This DKK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-45 amino acids from the N-terminal region of human DKK3.
NCBI Gene ID #:
27122
NCBI Official Name:
Dickkopf-related protein 3
NCBI Official Symbol:
DKK3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
38 kDa
Protein Accession #:
Q9UBP4
Protein GI Number:
311033372
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
Q9UBP4
User NOte:
Optimal dilutions for each application to be determined by the researcher.