ProSci

G2E3 Antibody

Product Code:
 
PSI-60-196
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in A549 cell line lysates (35ug/lane).
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Western blot analysis in A549 cell line lysates (35ug/lane).

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PSI-60-196-400ul400ul£626.00
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Further Information

Additional Names:
G2/M phase-specific E3 ubiquitin-protein ligase, 632-, G2E3, KIAA1333
Application Note:
For WB starting dilution is: 1:1000
Background:
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Essential in early embryonic development to prevent apoptotic death.
Background References:
  • Brooks, W.S., et al. J. Biol. Chem. 283(32):22304-22315(2008)
  • Brooks, W.S., et al. Exp. Cell Res. 313(4):665-676(2007)
  • Lehner, B., et al. Genomics 83(1):153-167(2004)
  • Crawford, D.F., et al. J. Biol. Chem. 276(40):37166-37177(2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This G2E3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-252 amino acids from the Central region of human G2E3.
NCBI Gene ID #:
55632
NCBI Official Name:
G2/M phase-specific E3 ubiquitin-protein ligase
NCBI Official Symbol:
G2E3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
81 kDa
Protein Accession #:
Q7L622
Protein GI Number:
74738611
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q7L622
User NOte:
Optimal dilutions for each application to be determined by the researcher.