ProSci

UBE2T Antibody

Product Code:
 
PSI-59-158
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of UBE2T using rabbit polyclonal UBE2T Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2T gene.
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Western blot analysis of UBE2T using rabbit polyclonal UBE2T Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2T gene.

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PSI-59-158-400ul400ul£626.00
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Further Information

Additional Names:
Ubiquitin-conjugating enzyme E2 T, Cell proliferation-inducing gene 50 protein, Ubiquitin carrier protein T, Ubiquitin-protein ligase T, UBE2T
Application Note:
For WB starting dilution is: 1:1000
Background:
The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 [PubMed 16916645]).
Background References:
  • Ueki, T., et al. Cancer Res. 69(22):8752-8760(2009)
  • Longerich, S., et al. J. Biol. Chem. 284(35):23182-23186(2009)
  • Alpi, A.F., et al. Mol. Cell 32(6):767-777(2008)
  • Hao, J., et al. Tumour Biol. 29(3):195-203(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This UBE2T antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-154 amino acids from the C-terminal region of human UBE2T.
NCBI Gene ID #:
29089
NCBI Official Name:
Ubiquitin-conjugating enzyme E2 T
NCBI Official Symbol:
UBE2T
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
23 kDa
Protein Accession #:
Q9NPD8
Protein GI Number:
73622065
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q9NPD8
User NOte:
Optimal dilutions for each application to be determined by the researcher.