ProSci

POTE Antibody

Product Code:
 
PSI-57-707
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with POTE antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
2 / 2
Western blot analysis of POTE Antibody in Ramos cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with POTE antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of POTE Antibody in Ramos cell line lysates (35ug/lane)

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PSI-57-707-400ul400ul£626.00
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Further Information

Additional Names:
POTE ankyrin domain family member D, ANKRD26-like family B member 3, Ankyrin repeat domain-containing protein 21, Prostate, ovary, testis-expressed protein, Protein POTE, POTED, A26B3, ANKRD21, POTE
Application Note:
For IHC-P starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
The POTE protein contains seven ankyrin repeats between amino acids 140 and 380. Expression of POTE in prostate cancer and its undetectable expression in normal essential tissues make POTE a candidate for the immunotherapy of prostate cancer. The existence of a large number of closely related but rapidly diverging members, their location on multiple chromosomes and their limited expression pattern suggest an important role for the POTE gene family in reproductive processes.
Background References:
  • Bera T.K., Proc. Natl. Acad. Sci. U.S.A. 99:16975-16980(2002).
  • Bera,T.K., Gene 337, 45-53 (2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This POTE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 542-571 amino acids from the C-terminal region of human POTE.
NCBI Gene ID #:
100288966, 317754
NCBI Official Name:
POTE ankyrin domain family member D
NCBI Official Symbol:
POTED
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
66 kDa
Protein Accession #:
Q86YR6
Protein GI Number:
269849632
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q86YR6
User NOte:
Optimal dilutions for each application to be determined by the researcher.