ProSci

SGPP1 Antibody

Product Code:
 
PSI-56-932
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western Blot at 1:1000 dilution + human placenta lysate Lysates/proteins at 20 ug per lane.
2 / 3
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
3 / 3
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Western Blot at 1:1000 dilution + human placenta lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).

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PSI-56-932-400ul400ul£626.00
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Further Information

Additional Names:
Sphingosine-1-phosphate phosphatase 1, SPPase1, Spp1, hSPP1, hSPPase1, 313-, Sphingosine-1-phosphatase 1, SGPP1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP1 catalyzes the degradation of S1P via salvage and recycling of sphingosine into long-chain ceramides (Mandala et al., 2000 [PubMed 10859351]; Le Stunff et al., 2007 [PubMed 17895250]).[supplied by OMIM].
Background References:
  • Hicks, A.A., et al. PLoS Genet.(10), E1000672 (2009) :
  • Le Stunff, H., et al. J. Biol. Chem. 282(47):34372-34380(2007)
  • Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SGPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human SGPP1.
NCBI Gene ID #:
81537
NCBI Official Name:
Sphingosine-1-phosphate phosphatase 1
NCBI Official Symbol:
SGPP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
49 kDa
Protein Accession #:
Q9BX95
Protein GI Number:
46577706
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q9BX95
User NOte:
Optimal dilutions for each application to be determined by the researcher.