ProSci

APIP Antibody

Product Code:
 
PSI-61-295
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Antibody staining APIP in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
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Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Raji whole cell lysate Lane 3: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
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Antibody staining APIP in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Raji whole cell lysate Lane 3: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.

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PSI-61-295-200ul200ul£626.00
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Further Information

Additional Names:
Methylthioribulose-1-phosphate dehydratase {ECO:0000255|HAMAP-Rule:MF_03116}, MTRu-1-P dehydratase {ECO:0000255|HAMAP-Rule:MF_03116}, 4.2.1.109 {ECO:0000255|HAMAP-Rule:MF_03116}, APAF1-interacting protein {ECO:0000255|HAMAP-Rule:MF_03116}, hAPIP, APIP {ECO:0000255|HAMAP-Rule:MF_03116}
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:2000
Background:
Catalyzes the dehydration of methylthioribulose-1- phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1- phosphate (DK-MTP-1-P). Functions in the methionine salvage pathway, which plays a key role in cancer, apoptosis, microbial proliferation and inflammation. May inhibit the CASP1-related inflammatory response (pyroptosis), the CASP9-dependent apoptotic pathway and the cytochrome c-dependent and APAF1-mediated cell death.
Background References:
  • Lai C.-H.,et al.Genome Res. 10:703-713(2000).
  • Ota T.,et al.Nat. Genet. 36:40-45(2004).
  • Taylor T.D.,et al.Nature 440:497-500(2006).
  • Cho D.-H.,et al.J. Biol. Chem. 279:39942-39950(2004).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This APIP antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 111-142 amino acids from the Central region of human APIP.
NCBI Gene ID #:
51074
NCBI Official Name:
Methylthioribulose-1-phosphate dehydratase {ECO:0000255|HAMAP-Rule:MF_03116}
NCBI Official Symbol:
APIP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
27 kDa
Protein Accession #:
Q96GX9
Protein GI Number:
74731866
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q96GX9
User NOte:
Optimal dilutions for each application to be determined by the researcher.