ProSci

PHPT1 Antibody

Product Code:
 
PSI-61-451
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of PHPT1 using rabbit polyclonal PHPT1 Antibody (Human C-term) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHPT1 gene (Lane 2).
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Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PHPT1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of PHPT1 using rabbit polyclonal PHPT1 Antibody (Human C-term) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHPT1 gene (Lane 2).
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PHPT1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-61-451-400ul400ul£626.00
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Further Information

Additional Names:
14 kDa phosphohistidine phosphatase, 313-, Phosphohistidine phosphatase 1, Protein janus-A homolog, PHPT1, PHP14
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
PHPT1 is an EDTA-insensitive phosphohistidine phosphatase that catalyzes the dephosphorylation of phosphopeptide I (Ek etal., 2002 [PubMed 12383260]).[supplied by OMIM].
Background References:
  • Lim,J., Cell 125 (4), 801-814 (2006)
  • Mehrle,A., Nucleic Acids Res. 34 (DATABASE ISSUE), D415-D418 (2006)
  • Ma,R.,Biochem. Biophys. Res. Commun. 337 (3), 887-891 (2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PHPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the C-terminal region of human PHPT1.
NCBI Gene ID #:
29085
NCBI Official Name:
14 kDa phosphohistidine phosphatase
NCBI Official Symbol:
PHPT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
14 kDa
Protein Accession #:
Q9NRX4
Protein GI Number:
25008934
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q9NRX4
User NOte:
Optimal dilutions for each application to be determined by the researcher.