ProSci

PSMA5 Antibody

Product Code:
 
PSI-57-770
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis of PSMA5 using rabbit polyclonal PSMA5 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PSMA5 gene (Lane 2).
2 / 3
Western blot analysis of PSMA5 Antibody in HL60 cell line lysates (35ug/lane). PSMA5 was detected using the purified polyclonal antibody.
3 / 3

Western blot analysis of PSMA5 using rabbit polyclonal PSMA5 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PSMA5 gene (Lane 2).
Western blot analysis of PSMA5 Antibody in HL60 cell line lysates (35ug/lane). PSMA5 was detected using the purified polyclonal antibody.

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PSI-57-770-400ul400ul£626.00
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Further Information

Additional Names:
Proteasome subunit alpha type-5, Macropain zeta chain, Multicatalytic endopeptidase complex zeta chain, Proteasome zeta chain, PSMA5
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PMA5 is a member of the peptidase T1A family, that is a 20S core alpha subunit.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This PSMA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106-135 amino acids from the Central region of human PSMA5.
NCBI Gene ID #:
5686
NCBI Official Name:
Proteasome subunit alpha type-5
NCBI Official Symbol:
PSMA5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
26 kDa
Protein Accession #:
P28066
Protein GI Number:
38258905
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle
Swissprot #:
P28066
User NOte:
Optimal dilutions for each application to be determined by the researcher.