ProSci

SPDYE3 Antibody

Product Code:
 
PSI-55-142
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis in A375 cell line lysates (35ug/lane).
2 / 3
SPDYE3 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cervical tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3
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Western blot analysis in A375 cell line lysates (35ug/lane).
SPDYE3 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cervical tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-55-142-400ul400ul£626.00
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Further Information

Additional Names:
Speedy protein E3, SPDYE3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background References:
  • Gerhard, D.S., et al. Genome Res. 14 (10B), 2121-2127 (2004) :
  • Hillier, L.W., et al. Nature 424(6945):157-164(2003)
  • Scherer, S.W., et al. Science 300(5620):767-772(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SPDYE3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 455-483 amino acids from the Central region of human SPDYE3.
NCBI Gene ID #:
441272
NCBI Official Name:
Speedy protein E3
NCBI Official Symbol:
SPDYE3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
64 kDa
Protein Accession #:
A6NKU9
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
A6NKU9
User NOte:
Optimal dilutions for each application to be determined by the researcher.