ProSci

WFDC12 Antibody

Product Code:
 
PSI-55-149
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis in A549 cell line lysates (35ug/lane).
2 / 3
WFDC12 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3
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Western blot analysis in A549 cell line lysates (35ug/lane).
WFDC12 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-55-149-400ul400ul£626.00
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Further Information

Additional Names:
WAP four-disulfide core domain protein 12, Putative protease inhibitor WAP12, Whey acidic protein 2, WFDC12, C20orf122, WAP2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
This gene encodes a member of the WAP-type four-disulfide core (WFDC) domain family. The WFDC domain, or WAP signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster.
Background References:
  • Clauss, A., et al. Biochem. Biophys. Res. Commun. 333(2):383-389(2005)
  • Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)
  • Clauss, A., et al. Biochem. J. 368 (PT 1), 233-242 (2002) :
  • Lundwall, A., et al. Biochem. Biophys. Res. Commun. 290(1):452-456(2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This WFDC12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-94 amino acids from the C-terminal region of human WFDC12.
NCBI Gene ID #:
128488
NCBI Official Name:
WAP four-disulfide core domain protein 12
NCBI Official Symbol:
WFDC12
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
12 kDa
Protein Accession #:
Q8WWY7
Protein GI Number:
24212610
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Immunology
Swissprot #:
Q8WWY7
User NOte:
Optimal dilutions for each application to be determined by the researcher.