ProSci

LGALS3 Antibody

Product Code:
 
PSI-56-128
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
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Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-56-128-400ul400ul£626.00
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Further Information

Additional Names:
Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, Galactose-specific lectin 3, Galactoside-binding protein, GALBP, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen, LGALS3, MAC2
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.
Background References:
  • Salomonsson, E., et al. J. Biol. Chem. 285(45):35079-35091(2010)
  • Debierre-Grockiego, F., et al. J. Biol. Chem. 285(43):32744-32750(2010)
  • Zhou, J.Y., et al. J. Proteome Res. 9(10):5133-5141(2010)
  • Markowska, A.I., et al. J. Exp. Med. 207(9):1981-1993(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This LGALS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-191 amino acids from the C-terminal region of human LGALS3.
NCBI Gene ID #:
3958
NCBI Official Name:
Galectin-3
NCBI Official Symbol:
LGALS3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
26 kDa
Protein Accession #:
P17931
Protein GI Number:
215274262
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Neuroscience
Swissprot #:
P17931
User NOte:
Optimal dilutions for each application to be determined by the researcher.