ProSci

CDK5R1 Antibody

Product Code:
 
PSI-63-179
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 2
Formalin-fixed and paraffin-embedded human brain tissue reacted with CDK5R1(p35) Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
2 / 2
Western blot analysis in A2058 cell line lysates (35ug/lane).
❮❯

Formalin-fixed and paraffin-embedded human brain tissue reacted with CDK5R1(p35) Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis in A2058 cell line lysates (35ug/lane).

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PSI-63-179-400ul400ul£626.00
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Further Information

Additional Names:
Cyclin-dependent kinase 5 activator 1, CDK5 activator 1, Cyclin-dependent kinase 5 regulatory subunit 1, TPKII regulatory subunit, Cyclin-dependent kinase 5 activator 1, p35, p35, Cyclin-dependent kinase 5 activator 1, p25, p25, Tau protein kinase II 23 kDa subunit, p23, CDK5R1, CDK5R, NCK5A
Application Note:
For IHC-P starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
p35 is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease.
Background References:
  • Lin,S., FEBS Lett. 582 (8), 1197-1202 (2008)
  • Sen,A., Neuroreport 18 (5), 511-516 (2007)
  • Mitsios,N., Brain Pathol. 17 (1), 11-23 (2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This CDK5R1(p35) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272-301 amino acids from the C-terminal region of human CDK5R1(p35).
NCBI Gene ID #:
8851
NCBI Official Name:
Cyclin-dependent kinase 5 activator 1
NCBI Official Symbol:
CDK5R1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
34 kDa
Protein Accession #:
Q15078
Protein GI Number:
2498217
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Neuroscience
Swissprot #:
Q15078
User NOte:
Optimal dilutions for each application to be determined by the researcher.