ProSci

UBA52 Antibody

Product Code:
 
PSI-55-070
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Western blot analysis in Hela cell line lysates (35ug/lane).
2 / 3
UBA52 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3
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Western blot analysis in Hela cell line lysates (35ug/lane).
UBA52 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

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PSI-55-070-400ul400ul£626.00
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Further Information

Additional Names:
Ubiquitin-60S ribosomal protein L40, CEP52, Ubiquitin A-52 residue ribosomal protein fusion product 1, Ubiquitin, 60S ribosomal protein L40, UBA52, UBCEP2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome.
Background References:
  • Memet, S. Biochem. Pharmacol. 72(9):1180-1195(2006)
  • Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006)
  • Foster, L.J., et al. J. Proteome Res. 5(1):64-75(2006)
  • Hu, Y., et al. Mol. Cell Proteomics 4(12):2000-2009(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Monkey, Mouse, Pig, Rat
Immunogen:
This UBA52 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-128 amino acids from the C-terminal region of human UBA52.
NCBI Gene ID #:
7311
NCBI Official Name:
Ubiquitin-60S ribosomal protein L40
NCBI Official Symbol:
UBA52
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
15 kDa
Protein Accession #:
P62987
Protein GI Number:
302393718
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Neuroscience
Swissprot #:
P62987
User NOte:
Optimal dilutions for each application to be determined by the researcher.