ProSci

YWHAB Antibody

Product Code:
 
PSI-63-434
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1 / 3
Antibody is used in Western blot to detect YWHAB in HL-60 cell lysate.
2 / 3
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
3 / 3
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Antibody is used in Western blot to detect YWHAB in HL-60 cell lysate.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

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PSI-63-434-400ul400ul£626.00
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Further Information

Additional Names:
14-3-3 protein beta/alpha, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1, 14-3-3 protein beta/alpha, N-terminally processed, YWHAB
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
YWHAB belongs to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for the gene. Both variants encode the same protein, however, they are differentially expressed in hematopoietic cells.
Background References:
  • Komori, T., et al., Acta Neuropathol. 106(1):66-70 (2003).
  • Cavet, M.E., et al., J. Biol. Chem. 278(20):18376-18383 (2003).
  • Li, Y., et al., J. Biol. Chem. 278(16):13663-13671 (2003).
  • Shumway, S.D., et al., J. Biol. Chem. 278(4):2089-2092 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Mouse, Rat
Immunogen:
This YWHAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-85 amino acids from the N-terminal region of human YWHAB.
NCBI Gene ID #:
7529
NCBI Official Name:
14-3-3 protein beta/alpha
NCBI Official Symbol:
YWHAB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
28 kDa
Protein Accession #:
P31946
Protein GI Number:
1345590
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Neuroscience,Signal Transduction
Swissprot #:
P31946
User NOte:
Optimal dilutions for each application to be determined by the researcher.