ProSci

PAX6 Antibody

Product Code:
 
PSI-62-543
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in U251 cell line lysates (35ug/lane).
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Formalin-fixed and paraffin-embedded human brain tissue with PAX6 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Flow cytometric analysis of hela cells using PAX6 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in U251 cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human brain tissue with PAX6 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of hela cells using PAX6 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-62-543-400ul400ul£626.00
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Further Information

Additional Names:
Paired box protein Pax-6, Aniridia type II protein, Oculorhombin, PAX6, AN2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
PAX6 is paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription.
Background References:
  • Firsova,N.V., et.al., Dokl. Biol. Sci. 426, 264-266 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat, Xenopus
Immunogen:
This PAX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-210 amino acids from the Central region of human PAX6.
NCBI Gene ID #:
5080
NCBI Official Name:
Paired box protein Pax-6
NCBI Official Symbol:
PAX6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
47 kDa
Protein Accession #:
P26367
Protein GI Number:
6174889
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Neuroscience,Signal Transduction
Swissprot #:
P26367
User NOte:
Optimal dilutions for each application to be determined by the researcher.