ProSci

PDF Antibody

Product Code:
 
PSI-60-274
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).
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Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).

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PSI-60-274-400ul400ul£626.00
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Further Information

Additional Names:
Peptide deformylase, mitochondrial, Polypeptide deformylase, PDF, PDF1A
Application Note:
For WB starting dilution is: 1:1000
Background:
Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells.
Background References:
  • Escobar-Alvarez, S., et al. J. Mol. Biol. 387(5):1211-1228(2009)
  • Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3558-3566(2008)
  • Lee, M.D., et al. J. Clin. Invest. 114(8):1107-1116(2004)
  • Serero, A., et al. J. Biol. Chem. 278(52):52953-52963(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PDF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-151 amino acids from the Central region of human PDF.
NCBI Gene ID #:
64146
NCBI Official Name:
Peptide deformylase, mitochondrial
NCBI Official Symbol:
PDF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
27 kDa
Protein Accession #:
Q9HBH1
Protein GI Number:
17433054
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
Q9HBH1
User NOte:
Optimal dilutions for each application to be determined by the researcher.