ProSci

HPD Antibody

Product Code:
 
PSI-63-537
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of HPD Antibody in mouse liver tissue lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HPD Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of HPD Antibody in mouse liver tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HPD Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-63-537-400ul400ul£626.00
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This product comes from: United States.
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Further Information

Additional Names:
4-hydroxyphenylpyruvate dioxygenase, 4-hydroxyphenylpyruvic acid oxidase, 4HPPD, HPD, HPPDase, HPD, PPD
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
HPD is an enzyme in the catabolic pathway of tyrosine. This protein catalyzes the conversion of 4-hydroxyphenylpyruvate to homogentisate.
Background References:
  • Item,C.B., et.al., Mol. Genet. Metab. 91 (4), 379-383 (2007)
  • Wistow,G., et.al., Mol. Vis. 8, 171-184 (2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This HPD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 255-284 amino acids from the C-terminal region of human HPD.
NCBI Gene ID #:
3242
NCBI Official Name:
4-hydroxyphenylpyruvate dioxygenase
NCBI Official Symbol:
HPD
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
45 kDa
Protein Accession #:
P32754
Protein GI Number:
417144
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
P32754
User NOte:
Optimal dilutions for each application to be determined by the researcher.