ProSci

AK2 Antibody

Product Code:
 
PSI-63-415
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:2000 dilution Lane 1: human heart lysate Lane 2: human kidney lysate Lane 3: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution Lane 1: human heart lysate Lane 2: human kidney lysate Lane 3: HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.

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PSI-63-415-400ul400ul£626.00
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Further Information

Additional Names:
Adenylate kinase 2, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03168}, AK 2 {ECO:0000255|HAMAP-Rule:MF_03168}, 2743 {ECO:0000255|HAMAP-Rule:MF_03168}, ATP-AMP transphosphorylase 2 {ECO:0000255|HAMAP-Rule:MF_03168}, ATP:AMP phosphotransferase {ECO:0000255|HAMAP-Rule:MF_03168}, Adenylate monophosphate kinase {ECO:0000255|HAMAP-Rule:MF_03168}, Adenylate kinase 2, mitochondrial, N-terminally processed {ECO:0000255|HAMAP-Rule:MF_03168}, AK2 {ECO:0000255|HAMAP-Rule:MF_03168}, ADK2
Application Note:
For WB starting dilution is: 1:2000

For IHC-P starting dilution is: 1:50~100
Background:
Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. Isozyme 2 is localized in the mitochondrial intermembrane space and may play a role in apoptosis.
Background References:
  • Noma, T., et al., Biochim. Biophys. Acta 1395(1):34-39 (1998).
  • Lee, Y., et al., J. Biochem. 123(1):47-54 (1998).
  • Lee, Y., et al., Biochem. Mol. Biol. Int. 39(4):833-842 (1996).
  • Bruns, G.A., et al., Biochem. Genet. 15 (5-6), 477-486 (1977).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This AK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-217 amino acids from the C-terminal region of human AK2.
NCBI Gene ID #:
204
NCBI Official Name:
Adenylate kinase 2, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03168}
NCBI Official Symbol:
AK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
26 kDa
Protein Accession #:
P54819
Protein GI Number:
1708596
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
P54819
User NOte:
Optimal dilutions for each application to be determined by the researcher.