ProSci

UGT2B15 Antibody

Product Code:
 
PSI-56-381
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in NCI-H460(lane 1),HepG2(lane 2),ZR-75-1(lane 3) cell line lysates (35ug/lane).
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UGT2B15 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in NCI-H460(lane 1),HepG2(lane 2),ZR-75-1(lane 3) cell line lysates (35ug/lane).
UGT2B15 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-56-381-400ul400ul£626.00
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Further Information

Additional Names:
UDP-glucuronosyltransferase 2B15, UDPGT 2B15, HLUG4, UDP-glucuronosyltransferase 2B8, UDPGT 2B8, UDPGTh-3, UGT2B15, UGT2B8
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
The UGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. UGT2B8 demonstrates reactivity with estriol. See UGT2B4 (MIM 600067).
Background References:
  • Yong, M., et al. Cancer Epidemiol. Biomarkers Prev. 19(2):537-546(2010)
  • Sun, C., et al. Hum. Mutat. 31(1):99-107(2010)
  • Ross, C.J., et al. Nat. Genet. 41(12):1345-1349(2009)
  • He, X., et al. BrClin Pharmacol 68(5):721-730(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This UGT2B15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-185 amino acids from the Central region of human UGT2B15.
NCBI Gene ID #:
7366
NCBI Official Name:
UDP-glucuronosyltransferase 2B15
NCBI Official Symbol:
UGT2B15
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
61 kDa
Protein Accession #:
P54855
Protein GI Number:
332278237
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle,Obesity,Signal Transduction
Swissprot #:
P54855
User NOte:
Optimal dilutions for each application to be determined by the researcher.