ProSci

PRELP Antibody

Product Code:
 
PSI-62-388
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis of PRELP antibody in Y79 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human hepatocarcinoma with PRELP Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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❮❯

Western blot analysis of PRELP antibody in Y79 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human hepatocarcinoma with PRELP Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

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PSI-62-388-400ul400ul£626.00
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Further Information

Additional Names:
Prolargin, Proline-arginine-rich end leucine-rich repeat protein, PRELP, SLRR2A
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
PRELP is a leucine-rich repeat protein present in connective tissue extracellular matrix. This protein functions as a molecule anchoring basement membranes to the underlying connective tissue. This protein has been shown to bind type I collagen to basement membranes and type II collagen to cartilage. It also binds the basement membrane heparan sulfate proteoglycan perlecan. This protein is suggested to be involved in the pathogenesis of Hutchinson-Gilford progeria (HGP), which is reported to lack the binding of collagen in basement membranes and cartilage.
Background References:
  • Grover,J., Matrix Biol. 26 (2), 140-143 (2007)
  • Johnson,J.M., Mol. Vis. 12, 1057-1066 (2006)
  • Lewis,M., Ageing Res. Rev.(1), 95-105 (2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PRELP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 257-286 amino acids from the C-terminal region of human PRELP.
NCBI Gene ID #:
5549
NCBI Official Name:
Prolargin
NCBI Official Symbol:
PRELP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
44 kDa
Protein Accession #:
P51888
Protein GI Number:
1709586
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle,Signal Transduction
Swissprot #:
P51888
User NOte:
Optimal dilutions for each application to be determined by the researcher.